Zebron ZB-5MSplus GC Columns
The Next Generation of Inertness
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Zebron ZB-5MSPLUS GC Columns
The Next Generation of Inertness
- The next generation of inertness for specialty chemical, forensic, toxicology, and food testing applications
- Specialized deactivation for versatile 5 % phenyl-arylene selectivity with improved sensitivity
- Low bleed (MS Certified) and well-suited to high sensitivity GC-MS and GC-MS/MS work
Running PAHs? See ZB-PAH-EU and ZB-PAH-CT
Running semivolatiles and PBDEs? See ZB-SemiVolatiles
Running at extremely high temperatures? See ZB-5HT Inferno
Recommended Use
Acids, Alkaloids, Amines, Drugs, Ethanolamines , Essential Oils/Flavors, Halo-hydrocarbons, Pesticides/Herbicides, Phenols, Residual Solvents, Solvent Impurities
Overview
Upgrade to Zebron from any 5 % phenyl or 5 % phenyl-arylene / 95 % dimethylpolysiloxane phase
- Agilent DB®-5ms
- Agilent DB-5ms Ultra Inert
- Agilent HP-5ms
- Agilent HP-5ms Ultra Inert
- Agilent VF-5msi
- Restek Rtx®-5Sil MS
- Supelco SLB®-5ms
Engineered for High Performance
Improved Inertness, Better Peak Shape, Low Bleed, and Aqueous Stability
Active sites on a GC column’s surface can result in analyte adsorption and degradation, negatively affecting peak shape and response. To reduce potential surface activity, Zebron ZB-5MSplus is designed with a rigorous fused silica deactivation process that improves inertness for troublesome compounds. Instantly achieve higher responses for active compounds compared to your current 5ms phase column, without changing your selectivity.
Melamine in Dog Food by GC-MS
Extraction Protocol:
- Combine 0.5 g of homogenized dog food with 10 mL of DEA/Water/Acetonitrile (1:4:5) in a 15 mL centrifuge tube
- Sonicate for 30 min
- Centrifuge at 5000 rpm for 10 min
- Transfer 100 µL of supernatant to an autosampler vial and evaporate to dryness using nitrogen gas
- Reconstitute with 100 µL of Acetonitrile/Pyridine (1:1) and then derivatize using 100 µL BSTFA with 1 % TCMS at 70 °C for 45 min
Column: | Zebron ZB-5MSplus |
Dimension: | 30 meter x 0.25 mm x 0.25 µm |
Part No.: | |
Injection Volume: | Splitless @ 280 °C, 1 µL |
Carrier Gas: | Helium @ 1 mL/min (constant flow) |
Oven Program: | 75 °C for 1 min to 320 °C @ 15 °C/min hold for 4 min |
Detector: | MSD @ 320 °C |
Sample:
1. Cyanuric acid
2. Ammelide
3. Ammeline
4. Melamine
5. Benzoguanamine
Versatile Performance For Drugs and Chemicals
Cannabinoids In Brownies by GC-MS
Extraction Protocol:
- Combine 1 g of chocolate brownie with 10 mL of water in a 50 mL centrifuge tube
- Shake using a mechanical shaker until dissolved
- Add roQ™ QuEChERS EN15662 extraction salt packet (KS0-8909) and 10 mL of acetonitrile
- Shake tube for 3 min using mechanical shaker
- Centrifuge at 2700 rpm for 5 min
Column: | Zebron ZB-5MSplus |
Dimension: | 30 meter x 0.25 mm x 0.25 µm |
Part No.: | |
Injection Volume: | Splitless @ 250 °C, 1 µL |
Carrier Gas: | Helium @ 1.8 mL/min (constant flow) |
Oven Program: | 100 °C for 1 min to 320 °C @ 50 °C/min,hold for 2 min |
Detector: | MSD @ 320 °C |
Sample:
1. Cannabidiol
2. ∆-9-Tetrahydrocannabinol
3. Cannabinol
Ethanolamines by GC-MS
Column: | Zebron ZB-5MSplus |
Dimension: | 30 meter x 0.25 mm x 1.00 µm |
Part No.: | |
Injection Volume: | Split 200:1 @ 250 °C, 1 µL |
Carrier Gas: | Helium @ 1.4 mL/min (constant flow) |
Oven Program: | 30 °C to 300 °C @ 40 °C/min |
Detector: | MSD @ 320 °C |
Sample:
1. Monoethanolamine
2. Diethanolamine
3. Triethylene glycol monomethyl ether (IS)
4. Triethanolamine
Documents
- Guide: GC Troubleshooting Quick Reference Guide
- Guide: Zebron GC Column Selection Chart
- Brochures: Zebron Gas Management Brochure
- Supporting Docs: Zebron Gas Filter Selection Tool
- Supporting Docs: Zebron Gas Filter User Guide
- Brochures: Phenova CRMs for Environmental Analysis Brochure
- Brochures: Chemical Industry LC & GC Separation Solutions Guide
- Technical Notes: Robust Analysis of Nitrosamine Using Strata Activated Carbon Extraction (AN-1110)
- Technical Notes: Ethanolamine Analysis by GC-MS using a Zebron ZB-5MSplus
- Guide: Persistent Organic Pollutants (POPs) by GC
- Brochures: GC FAQ - Troubleshooting Highlights from Webinar
- Technical Notes: Polycyclic Aromatic Hydrocarbons (PAHs) in Tattoo Ink by GC/MS (TN-2066)
FAQs
Can I pack or re-pack my GC liner with wool?
Though the upfront cost of self-packing your liner may seem attractive, the time and headaches caused by resulting tailing or irreproducible peaks can be sizeable! Self-packed wool fibers commonly break during installation and any existing deactivation on the liner can also be scratched or damaged. Scratches in the deactivation layer are likely to occur when removing wool from the liner or re-packing it with new wool. Also, when wool is forcefully inserted or removed, these scratches could expose active sites, resulting in peak tailing and poor reproducibility. Pre-packed Zebron PLUS liners undergo the deactivation process with the quartz wool already in place, which ensures that any active sites that form during packing are not exposed. Its novel touchless packaging and preinstalled O-ring prevent contamination during installation.
Does the Zebron PLUS liner with wool improve my GC column lifetime?
Yes. Zebron PLUS liners are packed with quartz wool, which serves two purposes: a) Quartz wool promotes homogenous vaporization of analytes and solvent in the inlet to effectively transfer them to the head of the GC column. b) Quartz wool acts as a filter, trapping non-volatile impurities and preventing them from proceeding further into the GC column.
I work with dirty samples which produce a residue on my GC liner after approximately 100 injections. Can I scrub/sonicate my liner and reuse it?
It is not recommended to scrub/sonicate your liners. Residue formation indicates contamination. More specifically, this shows that the sample contains non-volatile impurities, in which case it is recommended to replace your liner rather than clean it. Sonication can alter the liner’s deactivation, and scrubbing can create scratches and active sites. As a result, you can have several chromatography problems such as poor quantitation, analyte breakdown, poor reproducibility, peak asymmetry, etc.
Is there a way I can get immediate help with my current and upcoming method?
Absolutely, we're always here to help! You can easily get in contact with our team members by clicking contact us on the menu or better yet, just head to www.phenomenex.com/chatnow and we'll help you out with method recommendations and tips.
I am using an inert GC column for my analysis, and I still see peak tailing. What could be the reason for this?
For analysis of active compounds, it is crucial to use both a highly inert column and a highly inert liner. The liner is the first place of potential analyte interaction during GC analysis; it is important that your analytes are not adsorbed. Zebron PLUS liners undergo a rigorous deactivation process and are tested for inertness to ensure reliable results when working with highly active compounds such as underivatized acids and active bases.
When facing peak and resolution issues in sample analysis by GC, where is the best place to start?
The inlet is the most common place where problems happen in gas chromatography. This is because: • The inlet is subjected to very high temperatures. • There are multiple consumables involved in the inlet that need routine maintenance. • Some inlet modes, such as splitless injection, are a slow process, meaning that target analytes and the inlet can interact with each other before the sample enters the column.
Can you recommend a GC liner that work best when analyzing dirty samples, samples with wide boiling points, or samples with wide molecular weight?
Analysis of dirty samples, samples with wide boiling points, or samples with wide molecular weights can be enhanced by choosing a Zebron™ PLUS liner with quartz wool. Liners packed with wool can prevent non-volatile compounds from entering the column and improve sample vaporization for a more robust, reproducible analysis.
Manufacturer information: Zebron ZB-5MSPLUS GC Columns – Phenomenex (USA). MAS Brand is the official representative of Phenomenex in Egypt.
Specifications for Zebron ZB-5MSplus GC Columns
| GC Length (m) | 1.5 or 5 or 15 or 20 or 25 or 30 or 60 |
| GC Internal Diameter (mm) | 0.1 or 0.18 or 0.25 or 0.32 or 0.53 |
| GC Film Thickness (µm) | 0.1 or 0.18 or 0.25 or 0.36 or 0.5 or 1 or 1.5 or 2 |
| GC Format | Guard Column or Capillary Column or 5-inch Cage |
| GC Option | Standard or Custom or w/ GUARDIAN 10 m or w/ GUARDIAN 5 m |
| Manufacturer | Phenomenex |
| GC Phase | ZB-5MSplus |
