Synergi LC Columns
Full Range of Selectivity for Reversed Phase Separation
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Synergi HPLC Columns
Full Range of Selectivity for Reversed Phase Separation
Many different mechanisms of retention are utilized within reversed-phase chromatography in order to retain and separate target analytes. Whether your compounds are hydrophobic or polar, Synergi columns provide you with a full range of selectivity, ensuring the separation of the most challenging and complex mixtures.
| Separation technique | Reversed Phase (RP) |
|---|---|
| Application | Small Molecule Analysis |
| Pore size | 80 Å, 100 Å |
| Particle size | 2.5 µm, 4 µm, 10 µm |
| Phases available | 4 |
| Catalogue codes | 167 |
Full Range Selectivity
Synergi HPLC Columns four unique phases developed to provide a different selectivity for successful separations of the most complex mixtures and challenging analytes under reversed phase conditions.
Synergi Fusion-RP
C18 Polar Embedded
Balanced non-polar and polar performances
Synergi Hydro-RP
C18 Polar Embedded
Strong non-polar and polar retention
Synergi Max-RP
C12 TMS Endcapped
Excellent for basic compounds at neutral pH
Synergi Polar-RP
Phenyl Ether-Linked
For polar and aromatic mixtures
Phases
Synergi Fusion-RP
Polar embedded C18 with TMS endcapping
Recommended Use: Separation of mixtures with both polar and non-polar compounds
Particle Size: 10 µm, 4 µm, 2.5 µm
Synergi Hydro-RP
C18 with polar endcapping
Recommended Use: Separation of weakly hydrophobic compounds; 100% aqueous stable
Particle Size: 10 µm, 4 µm, 2.5 µm
Synergi Max-RP
C12 with TMS endcapping
Recommended Use: Separation of basic compounds under neutral pH
Particle Size: 10 µm, 4 µm, 2.5 µm
Synergi No Phase
Recommended Use: —
Particle Size: 10 µm, 4 µm, 2.5 µm
Synergi Polar-RP
Ether-linked phenyl with polar endcapping
Recommended Use: Separation of polar, aromatic compounds
Particle Size: 10 µm, 4 µm, 2.5 µm
Documents
- Supporting Docs: HPLC Column Care Guide
- Poster: HPLC/UHPLC Column Phase Selection Chart
- Supporting Docs: Frequently Asked Questions for UHPLC/HPLC
- Supporting Docs: Synergi Care and Use Notes
- Technical Notes: Sudan Dyes by HPLC-UV (TN-1028)
- Supporting Docs: Meeting All Your PREP Needs
- Supporting Docs: Meeting All Your SFC Needs
- Brochures: Pharmaceutical Analysis Guide, Volume 1 - Bioanalytical
- Brochures: roQ QuEChERS Food and Environmental Guide
- Brochures: Micro LC Columns and Traps Product Brochure
- Guide: Micro LC Columns - Care and Use Guide
- Technical Notes: Peptide Purification Method Development: Application for the Purification of Bivalirudin (TN-1129)
FAQs
What is chromatography?
Chromatography is the separation of components in a mixture based on their chemical nature. Chromatography can be performed in either liquid (HPLC) or gas (GC) forms. Liquid chromatography, specifically High Pressure Liquid Chromatography (HPLC), involves a stationary phase (sorbent), a mobile phase (liquid) and the analyte or compounds of interest. Analytes or compounds of interest are injected onto an HPLC column (packed with sorbent that contains a variety of chemistries) in mobile phase. As the mobile phase, which is carrying the analyte or compounds of interest, travels across the stationary phase, the analyte or compounds of interest separate from each other as they interact with the stationary phase, each eluting from the column at various times. Gas chromatography (GC) is similar to HPLC in that is utilized with working analytes or compounds of interest that can be vaporized without decomposing. In GC, gas is used instead of a liquid (like in HPLC). As analytes or compounds of interest travel in the gas mobile phase and interact with the stationary phase, eluting at various times.
Does high pH mobile phase reduce the sensitivity of basic analytes in LC-MS?
Using high pH mobile phase, the retention of polar bases will be increased. Signal response does depend upon ionization state, so if we use high pH, we are actually de-ionizing our bases and should lose sensitivity. But this doesn’t happen actually. When we increase the retention of the polar base analytes using high pH, it elutes later in the gradient run and in a high percentage of organic (acetonitrile or methanol). For example, when using formic acid, the polar base analyte peak is eluting in about 5-10% acetonitrile, but when we switch to pH 10, the polar base analyte peak is coming off in about 30-40% acetonitrile. The increased % of acetonitrile in the source is what allows the ions to be ionized thoroughly, thereby giving you a better response in ESI-MS.
What changes are allowed to USP / EP gradient HPLC methods?
When using a gradient method, no change may be made to particle size or column dimensions. They must be the same as those directed in the monograph.
I am looking to switch my pesticide methods from GC-MS to LC-MS, what do you recommend?
In general, a column with polar selectivity, such as Synergi Fusion-RP or Kinetex Biphenyl, will preform well for pesticide/herbicide screens using LC/MS/MS.
Manufacturer information: Synergi HPLC Columns – Phenomenex (USA). MAS Brand is the official representative of Phenomenex in Egypt.
Specifications for Synergi LC Columns
| Column Phase | MAX-RP or Max-RP or Hydro-RP or Polar-RP or Fusion-RP |
| Column Particle Size | 2.5 or 4 or 10 |
| Column Length | 10 or 20 or 30 or 50 or 75 or 100 or 125 or 150 or 250 or 300 or N/A |
| Column Internal Diameter | 0.3 or 0.5 or 1 or 2 or 3 or 3.9 or 4 or 4.6 or 10 or 21.2 or 30 or 50 or N/A |
| Column Pore Size | 80 or 100 |
| Column Format | MercuryMS or Bulk Media or Analytical or Capillary / Nano or Preparative (AXIA Packed) |
| Column Option | Standard or AXIA Packed or Capillary or MercuryMS or Titanium Frits |
| Manufacturer | Phenomenex |
