Lux LC Columns
One of the World's Leading Polysaccharide Columns for Chiral Separation
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Lux Chiral HPLC Columns
One of the World’s Leading Polysaccharide Columns for Chiral Separation
The Lux family of amylose and cellulose chiral selectors provides a variety of complementary selectivities that allows you to screen for the most effective enantiomeric separation under reversed phase, polar organic, normal phase, and SFC conditions.
| Separation technique | Chiral |
|---|---|
| Application | Small Molecule Analysis |
| Particle size | 3 µm, 5 µm |
| Phases available | 9 |
| Catalogue codes | 163 |
Overview
Simplified Chiral HPLC/SFC Column Screening Strategies
Lux immobilized chiral stationary phases provide complementary but distinct enantioselectivity for a wide range of chirality. In addition, the immobilization process allows for the use of a wide range of mobile phases and strong solvents compatibility
Immobilized
Coated
Application Guides
Phases
Lux AMP
Proprietary chiral column chemistry to separate substituted amphetamines
Recommended Use: Rapid and accurate chiral separation of methamphetamine and amphetamine.
Particle Size: 3 µm
Lux Amylose-1
Amylose tris(3,5-dimethylphenylcarbamate)
Recommended Use: Separation of enantiomers and racemic mixtures by reversed phase, normal phase, polar organic and SFC with a fully porous particle, coated with a polysaccharide stationary phase.
Particle Size: 5 µm, 3 µm
Lux Cellulose-1
Cellulose tris (3,5-dimethylphenylcarbamate)
Recommended Use: Separation of enantiomers and racemic mixtures by reversed phase, normal phase, polar organic and SFC with a fully porous particle, coated with a polysaccharide stationary phase.
Particle Size: 5 µm, 3 µm
Lux Cellulose-2
Cellulose tris (3-chloro-4-methylphenylcarbamate)
Recommended Use: Separation of enantiomers and racemic mixtures by reversed phase, normal phase, polar organic and SFC with a fully porous particle, coated with a polysaccharide stationary phase.
Particle Size: 5 µm, 3 µm
Lux Cellulose-3
Cellulose tris(4-methylbenzoate)
Recommended Use: Separation of enantiomers and racemic mixtures by reversed phase, normal phase, polar organic and SFC with a fully porous particle, coated with a polysaccharide stationary phase.
Particle Size: 5 µm, 3 µm
Lux Cellulose-4
Cellulose tris (4-chloro-3-methylphenylcarbamate)
Recommended Use: Separation of enantiomers and racemic mixtures by reversed phase, normal phase, polar organic and SFC with a fully porous particle, coated with a polysaccharide stationary phase.
Particle Size: 5 µm, 3 µm
Lux i-Amylose-1
Amylose tris(3,5-dimethylphenylcarbamate)
Recommended Use: Separation of enantiomers and racemic mixtures by reversed phase, normal phase, polar organic and SFC with a fully porous particle, immobilized with a polysaccharide stationary phase. This Immobilization provides additional strong organic solvent ruggedness so that DCM, DMSO and other related strong solvents can be used on column.
Particle Size: 5 µm
Lux i-Amylose-3
Amylose tris(3-chloro-5-methylphenylcarbamate)
Recommended Use: Separation of enantiomers and racemic mixtures with strong stability in strong organic solvents
Particle Size: 5 µm, 3 µm
Lux i-Cellulose-5
Cellulose tris(3,5-dichlorophenylcarbamate)
Recommended Use: Separation of enantiomers and racemic mixtures by reversed phase, normal phase, polar organic and SFC with a fully porous particle, immobilized with a polysaccharide stationary phase. This Immobilization provides additional strong organic solvent ruggedness so that DCM, DMSO and other related strong solvents can be used on column.
Particle Size: 5 µm, 3 µm
Lux No Phase
Recommended Use: —
Documents
- Supporting Docs: Lux Chiral Column Equivalency Flyer
- Brochures: The Chiral Notebook
- Guide: 2026 Product Catalog – Explore Full Range | Phenomenex
- Poster: Chiral Column Equivalence Chart
- Brochures: Lux AMP Column Product Guide & Benefits
- Brochures: United States Pharmacopeia (USP) Allowable Adjustments Guide
- Supporting Docs: Frequently Asked Questions for UHPLC/HPLC
- Supporting Docs: Meeting All Your PREP Needs
- Supporting Docs: Meeting All Your SFC Needs
- Brochures: Affordable Chiral Columns, Brilliant Separation Power
- Technical Notes: Isomeric Purity in Atomoxetine Hydrochloride per Ph. Eur. Monograph:2640 on a Lux 5 µm Cellulose-1 Column
- Technical Notes: Enantiomeric Purity of Rivaroxaban per Ph. Eur. Monograph:2932 on Lux Cellulose-1 Column
FAQs
What is chromatography?
Chromatography is the separation of components in a mixture based on their chemical nature. Chromatography can be performed in either liquid (HPLC) or gas (GC) forms. Liquid chromatography, specifically High Pressure Liquid Chromatography (HPLC), involves a stationary phase (sorbent), a mobile phase (liquid) and the analyte or compounds of interest. Analytes or compounds of interest are injected onto an HPLC column (packed with sorbent that contains a variety of chemistries) in mobile phase. As the mobile phase, which is carrying the analyte or compounds of interest, travels across the stationary phase, the analyte or compounds of interest separate from each other as they interact with the stationary phase, each eluting from the column at various times. Gas chromatography (GC) is similar to HPLC in that is utilized with working analytes or compounds of interest that can be vaporized without decomposing. In GC, gas is used instead of a liquid (like in HPLC). As analytes or compounds of interest travel in the gas mobile phase and interact with the stationary phase, eluting at various times.
How do I perform a solvent switch with Lux?
To safely transfer a Lux column from normal phase to polar organic or reverse phase conditions, flush the column with methanol:ethanol 9:1 (V/V) as transition solvent at a flow rate of 0.5 mL/min. First, flush the column with at least ten column volumes (i.e. 25 mL for a 250 x 4.6 mm i.d. column or 15 mL for a 150 x4.6 mm i.d. column) to completely remove the initial mobile phase. When the column has been flushed, equilibrate the column with at least ten column volumes of the polar organic or reverse phase solvent mixture to condition the column. In addition, when the buffer salt additive of the reverse phase mobile phase is insoluble in methanol/ethanol, flush the column briefly with water before switching to the buffered mobile phase. When the column has been flushed equilibrate the column with at least ten column volumes with the reverse phase solvent mixture. To safely transfer a column from polar organic to normal phase conditions flush the column with at least ten column volumes of methanol:ethanol 9:1 (V/V) as transition solvent at a flow rate of 0.5 mL/min. When the column has been flushed with methanol/ethanol equilibrate the column with at least ten column volume of the normal phase solvent mixture to condition the column. We do not recommend switching from reverse phase mode back to normal phase mode.
I have a Lux chiral column and I want to use it for SFC. Do I need to solvent switch?
No. Start with a lower flow rate than normal and pump CO2/MeOH (80/20). For example, if the method calls for 5 mL/min then start the initial flow into the column at 2-3 mL/min and gradually increase over about 20 minutes. AXIA preparative columns will require a longer ramp up to the normal flow rate (at least 30 minutes for a 21.2mm ID). The Hexane/IPA shipping solvent will be easily changed over to SFC mobile phase using this approach.
What solvents are Lux LC columns not compatible with?
Non-compatible solvents are DMSO, DMF, DCM, Acetone, THF, Ethyl acetate and any solvent capable of dissolving the polysaccharide derivative.
What is the difference between Amylose and Cellulose backbones?
While both form helical structures that are maintained in coating, the amylose can be described as more tightly coiled. The looser cellulose helical structure may more readily accommodate enantiomer interaction. Practically, this results in different selectivity even if the same chiral selector is substituted, as the 3-dimensional orientation around the CSP will differ.
How do aromatic functionalities in chiral compounds affect selectivity?
Chiral separations with Lux and Chirex are dependent on the nature of the aromatic groups in the chiral compounds of interest and the distance between the aromatic functionality and chiral center in the analyte. Generally, the closer the aromatic group is to the chiral center in a chiral compound the easier it is to separate it’s enantiomers and separations of chiral compounds where the chiral center is 4 or more atoms away from the aromatic ring are uncommon if not impossible. Stationary phases are most effective when the distances between the aromatic group and chiral center are the same in the chiral compound of interest. If the aromatic group of the compound has electron withdrawing groups like halogens or oxygen containing functionality, it will be more electron deficient and will interact more effectively with electron rich aromatic groups in the stationary phase (generally unsubstituted or with electron donating alkyl groups).
What separation modes do Lux Polysaccharide Chiral Columns operate in?
Lux columns are interchangeable between normal phase, polar organic, reversed phase, and SFC modes. Additionally, while Lux columns were designed for chiral separations they can be used under the same separation modes as well as HILIC for achiral separations.
How should I transfer a Lux chiral column from normal phase to SFC?
To safely transfer a column from normal phase to SFC, we recommend conditioning directly to 80:20 CO2:co-solvent (typically methanol) at a low pressure, increasing as the pressure stabilizes. You may also start with 100% CO2, considering the small amount of isopropanol (10%) in the shipping solvent is enough to overcome any concerns of straight CO2 displacement of straight hexane. 80:20 CO2:methanol is the equilibration solvent we use in our independent SFC analyses.
When making the mobile phase for the Lux AMP application, how much Ammonium Hydroxide do I use to adjust the 5 mM Ammonium Bicarbonate solution to pH 11?
Make up a 1 Liter solution of 5 mM Ammonium Bicarbonate and mix well. Transfer to a larger flask or beaker and follow the procedure. The solution will easily get to pH 9 with a small amount of concentrated Ammonium Hydroxide. In order to get to pH 11 you should expect to add an amount of concentrated Ammonium Hydroxide around 5-10% of the total volume of your solution. For example if you were to make a 1 Liter solution of 5 mM Ammonium Bicarbonate then expect to add as much as 50-100 mL of concentrated Ammonium Hydroxide.
How should I transfer a Lux chiral column from normal phase to polar organic or reversed phase?
This is quite a challenge as many of the products that we use, including our solvents, reagents, glassware, sample prep, and LC instrumentation can have PFAS background. Some general tips are to clean all equipment prior to, and after each use, make sure your reagent water, solvents such as methanol and ammonium hydroxide, are as PFAS free as possible. You may have to inquire from your vendor for details or if they can have some guarantee on background levels. There can also be PFAS in disposable plastics such as pipette tips and filters. Some methods recommend baking glassware, deterrent washing and rinsing with clean water, and proper storage for air drying. You can also implement a pre-rinse procedure using methanol with 0.1% ammonium hydroxide, toluene, and methanol. If you are using automated sample preparation, rinse the system with the same solvents. The LC pump should have a delay column between the pump and the autosampler as Teflon seals are common in LC systems. It’s advisable to review validated method guidance which often provide more detail on dealing with interferences within your lab, your system, and also within samples.
Manufacturer information: Lux Chiral HPLC Columns – Phenomenex (USA). MAS Brand is the official representative of Phenomenex in Egypt.
Specifications for Lux LC Columns
| Column Phase | AMP or Amylose-1 or Cellulose-1 or Cellulose-2 or Cellulose-3 or Cellulose-4 or i-Amylose-1 or i-Amylose-3 or i-Cellulose-5 |
| Column Particle Size | 3 or 5 |
| Column Length | 50 or 100 or 150 or 250 |
| Column Internal Diameter | 2 or 3 or 4.6 or 10 or 21.2 or 30 or 50 |
| Column Format | Analytical or Preparative (AXIA Packed) |
| Column Option | Standard or AXIA Packed |
| Manufacturer | Phenomenex |
