BioSep LC Columns
Aqueous Size Exclusion (SEC) / Gel Filtration (GFC) for Protein and Peptides
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BioSep GFC/SEC HPLC Columns
Aqueous Size Exclusion (SEC) / Gel Filtration (GFC) for Protein and Peptides
BioSep Gel Filtration (GFC/SEC) columns are used to analyze and/or characterize proteins, peptides, and other biomolecules, including antibodies, immunoglobulins, protein complexes, protein aggregates, and desalting. BioSep columns offer many important benefits to keep your research, method development/validation, and ongoing size exclusion separations simple. For new UHPLC/HPLC GFC method development, turn to Biozen SEC columns.
| Separation technique | Size Exclusion (SEC / GFC) |
|---|---|
| Application | Biological Analysis |
| Pore size | 145 Å, 290 Å, 500 Å |
| Particle size | 5 µm |
| Phases available | 3 |
| Catalogue codes | 12 |
Phases
BioSep SEC-s2000
Ideal for biogeneric aggregate applications, peptide therapeutics, small proteins, PEGylated peptides, and PEGylated small proteins.
Recommended Use: Hydrophilic bonded silica with a pore size of 145Å
Particle Size: 5 µm
BioSep SEC-s3000
Hydrophilic bonded silica with a pore size of 290Å
Recommended Use: Ideal for immunoglobulin aggregate applications, medium to large proteins, and serum proteins.
Particle Size: 5 µm
BioSep SEC-s4000
Hydrophilic bonded silica with a pore size of 500Å
Recommended Use: Ideal for large proteins and PEGylated IgG.
Particle Size: 5 µm
Documents
- Brochures: BioSep GFC/SEC Brochure
- Supporting Docs: HPLC Column Care Guide
- Guide: Protein and Peptide Users Guide
- Technical Notes: PEGylated Proteins by GFC (TN-1048)
- Technical Notes: PEGylated Proteins by GFC (TN-1082)
- Supporting Docs: Meeting All Your PREP Needs
- Technical Notes: Low Molecular Weight Separation by GFC (TN-1083)
- Brochures: Allowable Adjustments to European Pharmacopoeia (EP) Methods
- Supporting Docs: Certificate of Quality Assurance (CQA) Questions and Answers
- MSDS: Safety Data Sheet for Silica-Based HPLC Columns
- Brochures: Security Guard Column Protection Family Brochure (UHPLC, HPLC, PREP, SFC)
Manufacturer information: BioSep GFC/SEC HPLC Columns – Phenomenex (USA). MAS Brand is the official representative of Phenomenex in Egypt.
Specifications for BioSep LC Columns
| Column Phase | SEC-s2000 or SEC-s3000 or SEC-s4000 |
| Column Length | 30 or 35 or 75 or 300 or 600 |
| Column Internal Diameter | 4.6 or 7.8 or 21.2 |
| Column Pore Size | 145 or 290 or 500 |
| Column Format | Analytical or Guard (SecurityGuard) |
| Manufacturer | Phenomenex |
| Column Particle Size | 5 |
| Column Option | Standard |
