Zebron ZB-FAME GC Columns
Accelerating FAME Testing
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Zebron ZB-FAME GC Columns
The Fast FAME GC Column
- Reduce run times for traditional FAMEs separations up to 75 %
- Separate cis/trans FAME isomers with Rs ≥ 1.0
- High-cyanopropyl selectivity acceptable for use with AOCS, AOAC, and IOC methods
Application Notes:
- Reproducible FAME Analysis on a 100 meter Zebron™ ZB-FAME GC Column. Read more.
- Demonstration of Robustness and Selectivity for the Analysis of FAMEs in Salmon Oil and Pet Food. Read more.
Recommended Use
Fatty acid methyl esters (FAMEs), cis/trans FAME isomers, Omega 3 and Omega 6 FAMES
Overview
Upgrade to Zebron from any high cyanopropyl phase:
Agilent® | Supelco® |
|---|---|
| CP-Sil 88 | SP®-2380 |
| HP-88 | SP-2560 |
DB®-23 |
Accelerating FAME Testing
Designed to move beyond the conventional to the exceptional , Zebron GC columns come to life through a coupling of innovative spirit and technical excellence. As part of the Zebron Unlimited series of columns, Zebron ZB FAME offers unparalleled performance through the power of targeted selectivity when analyzing fatty acid methyl esters (FAMEs)
Optimized High-Cyano Polarity
FAME separations require the use of high polarity columns for adequate resolution of cis/trans isomers. Zebron™ ZB-FAME is an optimized high-cyanopropyl (G48) chemistry that provides the polarity that you need, while remaining compatible with AOAC, OCS, and IOC regulatory methods.
Elution Order for 37 FAME Standard
Peak | Compound | ID |
|---|---|---|
| 1 | Butanoic Acid Methyl Ester | C4:0 |
| 2 | Hexanoic Acid Methyl Ester2 | C6:0 |
| 3 | Octanoic Acid Methyl Ester | C8:0 |
| 4 | Decanoic Acid Methyl Ester | C10:0 |
| 5 | Undecanoic Acid Methyl Ester | C11:0 |
| 6 | Dodecanoic Acid Methyl Ester | C12:0 |
| 7 | Tridecanoic Acid Methyl Ester | C13:0 |
| 8 | Myristic Acid Methyl Ester | C14:0 |
| 9 | Myristoleic Acid Methyl Ester | C14:1 cis 9 |
| 10 | Pentadecanoic Acid Methyl Ester | C15:0 |
| 11 | cis-10-Pentadecenoic Acid Methyl Ester | C15:1 cis 10 |
| 12 | Hexadecanoic Acid Methyl Ester | C16:0 |
| 13 | Palmitoleic Acid Methyl Ester | C16:1 cis 9 |
| 14 | Heptadecanoic Acid Methyl Ester | C17:0 |
| 15 | cis-10-Heptadecenoic Acid Methyl Ester | C17:1 cis 10 |
| 16 | Stearic Acid Methyl Ester | C18:0 |
| 17 | Elaidic Acid Methyl Ester | C18:1 trans 9 |
| 18 | Oleic Acid Methyl Ester | C18:1 cis 9 |
| 19 | Linolelaidic Acid Methyl Ester | C18:2 trans 9,12 |
Peak | Compound | ID |
|---|---|---|
| 1 | Linoleic Acid Methyl Ester | C18:2 cis 9,12 |
| 2 | γ-Linolenic Acid Methyl Ester | C18:3 cis 6,9,12 |
| 3 | α-Linolenic Acid Methyl Ester | C18:3 cis 9,12,15 |
| 4 | Arachidic Acid Methyl Ester | C20:0 |
| 5 | cis-11-Eicosenoic Acid Methyl Ester | C20:1 cis 11 |
| 6 | cis-11,14-Eicosadienoic Acid Methyl Ester | C20:2 cis 11,14 |
| 7 | Heneicosanoic Acid Methyl Ester | C21:0 |
| 8 | cis-8,11,14-Eicosatrienoic Acid Methyl Ester | C20:3 cis 8,11,14 |
| 9 | Arachidonic Acid Methyl Ester | C20:4 cis 5,8,11,14 |
| 10 | cis-11,14,17-Eicosatrienoic Acid Methyl Ester | C20:3 cis 11,14,17 |
| 11 | Behenic Acid Methyl Ester | C22:0 |
| 12 | Erucic Acid Methyl Ester | C22:1 cis 13 |
| 13 | cis-5,8,11,14,17-Eicosapentaenoic Acid Methyl Ester | C20:5 cis 5,8,11,14,17 |
| 14 | cis-13,16-Docosadienoic Acid Methyl Ester | C22:2 cis 13,16 |
| 15 | Tricosanoic Acid Methyl Ester | C23:0 |
| 16 | Lignoceric Acid Methyl Ester | C24:0 |
| 17 | Nervonic Acid Methyl Ester | C24:1 cis 15 |
| 18 | cis-4,7,10,13,16,19-Docosahexaenoic Acid Methyl Ester | C22:6 cis 4,7,10,13,16,19 |
QC Tested For FAMEs
Baseline Separation of Common Isomers
Food and dietary standards require separation of cis/trans fatty acids. Zebron™ ZB-FAME is able to separate from C4 to C24 with resolution of specific cis/trans fatty acids.
Completely Resolve AOAC Critical Pairs
Columns: | Zebron ZB-FAME |
Dimension: | 30 meter x 0.25 mm x 0.20 µm |
Detector: | FID @ 260 °C |
Part No.: | 7HG-G033-10 |
Injection: | Split 50:1 @ 240 °C, 1 µL |
Recommended Liner: | Zebron PLUS Single Taper with Wool |
Liner Part No.: | AG2-0A11-05 (for Agilent® systems) |
Carrier Gas: | Helium @ 1.2 mL/min (constant flow) |
Oven Program: | 100 °C for 2 min to 140 °C @ 10 °C/minto 190 °C @ 3 °C/min to 260 °C @ 30 °C/min for 2 min |
Sample:
37 FAME standard
Separate Detailed C18 Compounds
Due to regulatory guidelines around trans fat content in food, detailed analysis of specific FAMEs isomers is commonly performed. The below methods demonstrate this testing using a 60 meter Zebron™ ZB-FAME column.
Analysis of C18:1, C18:2, and C18:3
Columns: | Zebron ZB-FAME |
Dimension: | 60 meter x 0.25 mm x 0.20 µm |
Detector: | FID @ 260 °C |
Part No.: | 7KG-G033-10 |
Injection: | Split 50:1 @ 240 °C, 1 µL |
Recommended Liner: | Zebron PLUS Single Taper with Wool |
Liner Part No.: | AG2-0A11-05 (for Agilent® systems) |
Carrier Gas: | Helium @ 30 cm/sec (constant flow) |
Oven Program: | 160 °C (isothermal) |
Sample:
As listed. For full peak identification, visit www.phenomenex.com/DetailedFAME
Twice The Runs, Half The Time
37 FAMEs In A Short 11 Minute Run
Traditionally, cis/trans FAME separations require the use of long (100 meters or more) columns and can run up to 60 minutes, resulting in a bottleneck to higher productivity. Zebron™ ZB-FAME provides targeted selectivity that allows for reduced column length – run times as short as 11 minutes without compromising your results!
Columns: | Zebron ZB-FAME |
Dimension: | 20 meter x 0.18 mm x 0.15 μm |
Detector: | FID @ 260 °C |
Part No.: | 7FD-G033-05 |
Injection: | Split 100:1 @ 250 °C, 1 µL |
Recommended Linear: | Zebron PLUS Single Taper Z-Liner™ |
Liner Part No.: | AG2-0A13-05 (for Agilent® systems) |
Carrier Gas: | Helium @ 1.0 mL/min (constant flow) |
Oven Program: | 80 °C for 1.5 min to 160 °C @ 40 °C/min to 185 °C @ 5 °C/min to 260 °C @ 30 °C/min |
Sample:
37 FAME standard
Documents
- Guide: GC Troubleshooting Quick Reference Guide
- Technical Notes: Fast Analysis of 37 FAMEs in 11 Minutes Using a Zebron ZB-FAME GC Column
- Technical Notes: 37 FAMEs In Under 25 Minutes by GC/FID Using Zebron ZB-FAME
- Technical Notes: Optimized Column Selectivity for Orthogonal Separation of Fatty Acid Methyl Esters (FAMEs) Using GCxGC (TN-2064)
- Technical Notes: FAMEs In Peanut Butter by GC/FID Using Zebron ZB-FAME
- Technical Notes: FAMEs In Monterey Jack Cheese by GC/FID Using Zebron ZB-FAME
- Technical Notes: Robust FAME Analysis in Salmon Oil & Pet Food with Zebron ZB-FAME Column
- Supporting Docs: Zebron Gas Filter User Guide
- Guide: Zebron GC Column Selection Chart
- Supporting Docs: Zebron Gas Filter Selection Tool
- Brochures: Zebron Gas Management Brochure
- Guide: Analyzing Fats and Oils by GC
FAQs
Can I pack or re-pack my GC liner with wool?
Though the upfront cost of self-packing your liner may seem attractive, the time and headaches caused by resulting tailing or irreproducible peaks can be sizeable! Self-packed wool fibers commonly break during installation and any existing deactivation on the liner can also be scratched or damaged. Scratches in the deactivation layer are likely to occur when removing wool from the liner or re-packing it with new wool. Also, when wool is forcefully inserted or removed, these scratches could expose active sites, resulting in peak tailing and poor reproducibility. Pre-packed Zebron PLUS liners undergo the deactivation process with the quartz wool already in place, which ensures that any active sites that form during packing are not exposed. Its novel touchless packaging and preinstalled O-ring prevent contamination during installation.
Does the Zebron PLUS liner with wool improve my GC column lifetime?
Yes. Zebron PLUS liners are packed with quartz wool, which serves two purposes: a) Quartz wool promotes homogenous vaporization of analytes and solvent in the inlet to effectively transfer them to the head of the GC column. b) Quartz wool acts as a filter, trapping non-volatile impurities and preventing them from proceeding further into the GC column.
I work with dirty samples which produce a residue on my GC liner after approximately 100 injections. Can I scrub/sonicate my liner and reuse it?
It is not recommended to scrub/sonicate your liners. Residue formation indicates contamination. More specifically, this shows that the sample contains non-volatile impurities, in which case it is recommended to replace your liner rather than clean it. Sonication can alter the liner’s deactivation, and scrubbing can create scratches and active sites. As a result, you can have several chromatography problems such as poor quantitation, analyte breakdown, poor reproducibility, peak asymmetry, etc.
Is there a way I can get immediate help with my current and upcoming method?
Absolutely, we're always here to help! You can easily get in contact with our team members by clicking contact us on the menu or better yet, just head to www.phenomenex.com/chatnow and we'll help you out with method recommendations and tips.
I am using an inert GC column for my analysis, and I still see peak tailing. What could be the reason for this?
For analysis of active compounds, it is crucial to use both a highly inert column and a highly inert liner. The liner is the first place of potential analyte interaction during GC analysis; it is important that your analytes are not adsorbed. Zebron PLUS liners undergo a rigorous deactivation process and are tested for inertness to ensure reliable results when working with highly active compounds such as underivatized acids and active bases.
When facing peak and resolution issues in sample analysis by GC, where is the best place to start?
The inlet is the most common place where problems happen in gas chromatography. This is because: • The inlet is subjected to very high temperatures. • There are multiple consumables involved in the inlet that need routine maintenance. • Some inlet modes, such as splitless injection, are a slow process, meaning that target analytes and the inlet can interact with each other before the sample enters the column.
Can you recommend a GC liner that work best when analyzing dirty samples, samples with wide boiling points, or samples with wide molecular weight?
Analysis of dirty samples, samples with wide boiling points, or samples with wide molecular weights can be enhanced by choosing a Zebron™ PLUS liner with quartz wool. Liners packed with wool can prevent non-volatile compounds from entering the column and improve sample vaporization for a more robust, reproducible analysis.
Manufacturer information: Zebron ZB-FAME GC Columns – Phenomenex (USA). MAS Brand is the official representative of Phenomenex in Egypt.
Specifications for Zebron ZB-FAME GC Columns
| GC Length (m) | 20 or 30 or 60 or 100 |
| GC Internal Diameter (mm) | 0.18 or 0.25 |
| GC Film Thickness (µm) | 0.15 or 0.2 |
| GC Format | Capillary Column or 5-inch Cage |
| GC Option | Standard or w/ GUARDIAN 5 m |
| Manufacturer | Phenomenex |
| GC Phase | ZB-FAME |
