Zebron ZB-35 GC Columns
Low Bleed, Intermediate Polarity Selectivity for GC-MS
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Zebron ZB-35 GC Columns
Low Bleed, Intermediate Polarity Selectivity for GC-MS
- Intermediate polarity column for high molecular weight analysis
- Inert to minimize analyte adsorption, improve efficiency, and reproducibility
- More rugged (longer column life) than other polar phases
- Excellent for trace analysis with bleed-sensitive detectors (MS, FID, ECD, NPD)
Running at high temperatures? See ZB-35HT-Inferno
Recommended Use
Amines, Aroclors, Drugs, EPA Methods (508, 608, 8081, 8141, 8151), Pesticides, Pharmaceuticals, Semi-volatiles, Steroids
Overview
Upgrade to Zebron from any 35 % phenyl / 65 % dimethylpolysiloxane phase:
Agilent® | Restek® | SGE® | Supelco® | OV® |
DB®-35 | Rtx®-35 | BP1BPX1-SimDBPX3 BPX608 | MDN-35SPB®-35SPB-608 | OV-11 |
Common Drug Screen by GC-FID
Column: | Zebron ZB-35 |
Dimension: | 30 meter x 0.25 mm x 0.25 µm |
Part No.: | |
Injection: | Split 10:1 @ 225 °C, 1.5 µL |
Carrier Gas: | Helium @ 1.4 mL/ min (constant flow) |
Oven Program: | 35 °C to 430 °C @ 9 °C/min for 10 min |
Detection: | 120 ˚C to 180 ˚C @ 25 ˚C/min to 200 ˚C @ 6 ˚C/min to 300 ˚C @ 20 ˚C/min for 3 min |
Detector: | FID @ 300 °C |
Sample:
All analytes are 25 ppm except nicotine at 100 ppm
1. Nicotine
2. Ibuprofen
3. Allobarbital
4. Acetaminophen
5. Aprobarbital
6. Butalbital
7. Amobarbital
8. Pentobarbital
9. Phenacetin
10. Secobarbital
11. Benzphetamine
12. Meprobamate
13. Dimenhydrinate
14. Hexobarbital
15. Doxylamine
16. Caffeine
17. Chlorpheniramine
18. Methapyrilene
19. Phenobarbital
20. Procaine
21. Bromopheniramine
22. Chlorcyclizine
23. Cocaine
24. Benactyzine
25. Codeine
26. Diazepam
27. Morphine
28. Hydrocodone
29. Oxymorphone
30. Heroin
Documents
- Supporting Docs: Zebron Essentials Phases
- Brochures: Zebron Essentials Brochure
- Guide: GC Troubleshooting Quick Reference Guide
- Guide: Zebron GC Column Selection Chart
- Brochures: Zebron Gas Management Brochure
- Supporting Docs: Zebron Gas Filter User Guide
- Supporting Docs: Zebron Gas Filter Selection Tool
- Brochures: Chemical Industry LC & GC Separation Solutions Guide
FAQs
Can I pack or re-pack my GC liner with wool?
Though the upfront cost of self-packing your liner may seem attractive, the time and headaches caused by resulting tailing or irreproducible peaks can be sizeable! Self-packed wool fibers commonly break during installation and any existing deactivation on the liner can also be scratched or damaged. Scratches in the deactivation layer are likely to occur when removing wool from the liner or re-packing it with new wool. Also, when wool is forcefully inserted or removed, these scratches could expose active sites, resulting in peak tailing and poor reproducibility. Pre-packed Zebron PLUS liners undergo the deactivation process with the quartz wool already in place, which ensures that any active sites that form during packing are not exposed. Its novel touchless packaging and preinstalled O-ring prevent contamination during installation.
Does the Zebron PLUS liner with wool improve my GC column lifetime?
Yes. Zebron PLUS liners are packed with quartz wool, which serves two purposes: a) Quartz wool promotes homogenous vaporization of analytes and solvent in the inlet to effectively transfer them to the head of the GC column. b) Quartz wool acts as a filter, trapping non-volatile impurities and preventing them from proceeding further into the GC column.
I work with dirty samples which produce a residue on my GC liner after approximately 100 injections. Can I scrub/sonicate my liner and reuse it?
It is not recommended to scrub/sonicate your liners. Residue formation indicates contamination. More specifically, this shows that the sample contains non-volatile impurities, in which case it is recommended to replace your liner rather than clean it. Sonication can alter the liner’s deactivation, and scrubbing can create scratches and active sites. As a result, you can have several chromatography problems such as poor quantitation, analyte breakdown, poor reproducibility, peak asymmetry, etc.
Is there a way I can get immediate help with my current and upcoming method?
Absolutely, we're always here to help! You can easily get in contact with our team members by clicking contact us on the menu or better yet, just head to www.phenomenex.com/chatnow and we'll help you out with method recommendations and tips.
I am using an inert GC column for my analysis, and I still see peak tailing. What could be the reason for this?
For analysis of active compounds, it is crucial to use both a highly inert column and a highly inert liner. The liner is the first place of potential analyte interaction during GC analysis; it is important that your analytes are not adsorbed. Zebron PLUS liners undergo a rigorous deactivation process and are tested for inertness to ensure reliable results when working with highly active compounds such as underivatized acids and active bases.
When facing peak and resolution issues in sample analysis by GC, where is the best place to start?
The inlet is the most common place where problems happen in gas chromatography. This is because: • The inlet is subjected to very high temperatures. • There are multiple consumables involved in the inlet that need routine maintenance. • Some inlet modes, such as splitless injection, are a slow process, meaning that target analytes and the inlet can interact with each other before the sample enters the column.
Can you recommend a GC liner that work best when analyzing dirty samples, samples with wide boiling points, or samples with wide molecular weight?
Analysis of dirty samples, samples with wide boiling points, or samples with wide molecular weights can be enhanced by choosing a Zebron™ PLUS liner with quartz wool. Liners packed with wool can prevent non-volatile compounds from entering the column and improve sample vaporization for a more robust, reproducible analysis.
Manufacturer information: Zebron ZB-35 GC Columns – Phenomenex (USA). MAS Brand is the official representative of Phenomenex in Egypt.
Specifications for Zebron ZB-35 GC Columns
| GC Length (m) | 10 or 15 or 25 or 30 or 60 or 12.5 |
| GC Internal Diameter (mm) | 0.1 or 0.25 or 0.53 or 0.32 or 0.2 |
| GC Film Thickness (µm) | 1 or 0.1 or 0.25 or 0.33 or 0.5 |
| GC Format | Capillary Column or 5-inch Cage |
| GC Option | Standard or Custom or w/ GUARDIAN 5 m, Custom |
| Manufacturer | Phenomenex |
| GC Phase | ZB-35 |
