Zebron ZB-WAXplus GC Columns
WAX columns from different manufacturers are not always created equal
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Zebron ZB-WAXplus GC Columns
100% Aqueous Stability for Polar Compounds
- 100% aqueous stable, excellent for aqueous samples; bonded and solvent rinseable
- Extremely inert for acidic compounds
- Enhanced selectivity for low boiling solvents; high retention of alcohols and chlorinated solvents
- Increased efficiency at 20 °C
Need a G43 phase for residual solvents testing? See ZB-624.
Testing free fatty acids? See ZB-FFAP.
Recommended Applications
Alcohols, Aldehydes, Aromatics, Essential Oils, Flavors & Fragrances, Free Fatty Acids, Glycols, OVIs, Pharmaceuticals, Solvents / Residual Solvents, Styrene, Xylene Isomers
Overview
Zebron ZB WAXPLUS GC Column
WAX columns from different manufacturers are not always created equal!
As part of the Zebron PLUS GC column family, ZB-WAX PLUS is manufactured using improved technologies that reflect our attention to detail every aspect of a ZB-WAX PLUS column’s anatomy is meticulously engineered and individually tested for unprecedented performance.
Upgrade to Zebron from any polyethylene glycol phase:
- Agilent DB-WAX
- Agilent CAM
- Agilent HP-20M
- Agilent Carbowax 20M
- Agilent CP-Wax 52 CB
- Restek Stabilwax
- SGE BP20
- SUPELCOWAX 10
Water Reproducibility of ZB-WAXPLUS
Historically, polyethylene glycol (PEG) phases have been unstable with aqueous samples such as beverages or glycols, resulting in poor reproducibility and decreased lifetime. The ZB-WAXplus bonding procedure results in exceptional stability to repeated injections of aqueous matrices.
Reproducibility and Stability Test
Columns: | Zebron ZB-WAXPLUS |
Dimension: | 30 meter x 0.25 mm x 0.25 µm |
Detector: | FID @ 200 °C |
Part No.: | 7HG-G013-11 |
Injection: | Split 30:1 @ 140 °C, 0.2 µL |
Carrier Gas: | Helium @ 1.4 mL/min (constant flow) |
Oven Program: | 35 °C for 5 min to 85 °C @ 10 °C/min to 200 °C @ 25 °C/min for 1 min |
Sample:
- Acetaldehyde
- Ethyl Acetate
- Methanol
- Ethanol
- Propanol
- Isobutanol
- 2-Methylbutanol
- 3-Methylbutanol
ZB-WAXPLUS provides optimal selectivity for many aqueous soluble compounds such as those found in alcoholic beverages or glycol samples. The ZB WAXPLUS bonding process results in exceptional stability to repeated injections of aqueous matrices like whiskey. Multiple or routine water injections will not alter the efficiency or polarity of the phase over time.
Distilled Alcohol Standard by Neat Injection on GC-FID
Columns: | Zebron ZB-WAXPLUS |
Dimension: | 30 meter x 0.25 mm x 0.25 µm |
Part No.: | 7HG-G013-11 |
Injection: | Split 25:1 @ 210 °C, 1 µL |
Carrier Gas: | Hydrogen @ 1 mL/min (constant flow) |
Oven Program: | 35 °C for 6 min to 60 °C @ 5 °C/min for 2 min to 210 °C @ 10 °C/min |
Detector: | FID @ 230 °C |
Note: | 200 ppm standard in methylenechloride |
Sample
1. Acetaldehyde
2. Isobutanal
3. Ethyl formate
4. Acrolein
5. Ethyl acetate
6. Acetal
7. Methanol
8. Methylene chloride
9. Ethanol
10. Isobutyl acetate
11. 2-Butanol
12. Ethyl butyrate
13. 1-Propanol
14. Isobutanol
15. Allyl alcohol
16. Isoamyl acetate
17. 1-Butanol
18. 4-Methyl-2-pentanol
19. Methyl-2-butanol
20. Methyl-3-butanol
21. Ethyl caproate
22. Ethyl heptanoate
23. Ethyl lactate
24. Hexanol
25. cis-3-Hexenol
26. Ethyl caprylate
27. Furfural
28. Benzaldehyde
29. Linalool
30. Linalyl acetate
31. Ethyl caprate
32. Diethyl succinate
33. Ethyl laurate
34. 2-Phenyl ethanol
Don’t Despair – There’s a Solution!
Low column activity is essential to achieving good quantitation and sensitivity. Whether you are analyzing aldehydes, acids, or pharmaceutical compounds the ZB-WAXPLUS provides the highest level of deactivation possible.
Conditions same for both columns:
Dimension: | 30 meter x 0.25 mm x 0.25 μm |
|---|---|
Detector: | FID @ 225 °C |
Part No.: | 7HG-G013-11(ZB-WAXPLUS) |
Injection Volume: | Split 100:1 @ 250 °C, 1 μL |
Carrier Gas: | Hydrogen @ 1.0 mL/min (constant flow) |
Oven Program: | 40 °C for 5 min to 200 °C at 10 °C/min and hold until last peak elutes |
Sample:
- Propanal
- Butanal
- Pentanal
- Hexanal
- Heptanal
- Octanal
- Nonanal
- Decanal
- Undecanal
Holds Up To Tough Conditions
In addition to inertness, ZB-WAXPLUS columns maintain good performance with difficult matrices and contaminants! The example below shows good results with glycols from seawater, despite the salt and other contaminants commonly found in this matrix.
Glycols From Seawater By GC-MS
Analytical Procedure
Sample Preparation
SPE Sorbent: | Strata®-X-A 500 mg / 6 mL |
|---|---|
Condition: | 3 mL Methanol, 3 mL Water |
Part No.: | 8B-S123-HCH |
Load: | 1 mL seawater |
Dry: | Full vacuum 5-10 minutes |
Elute: | 3 mL or 2 % Formic acid in Methanol |
GC/MS Conditions
Column: | Zebron ZB-WAXPLUS |
|---|---|
Dimension: | 30 meter x 0.25 mm x 0.25 µm |
Detection: | MSD, SIM from 1-2.8 min mass 87 and 89, 2.8-5 min mass 31 |
Part No.: | 7HG-G013-11 |
Injection: | Split 10:1 @ 250 °C, 1 µL |
Carrier: | Helium @ 1.5 mL/min (constant flow) |
Oven Program: | 100 °C to 200 °C @ 20 °C/min, post run 5 min @ 250 °C |
Sample:
Analytes were extracted from seawater (1 mL load @ 5 ppm)
- D4-2-Butoxyethanol (IS)
- 2-Butoxyethanol
- Formic acid (from elution)
- Propylene glycol
- Ethylene glycol (IS)
Improve Resolution
Conditions same for both columns:
Dimension: | 30 meter x 0.25 mm x 0.25 µm |
|---|---|
Part No.: | 7HG-G013-11 (ZB-WAXPLUS) |
Detector: | FID @ 225 °C |
Injection: | Split 100:1 @ 250 °C, 1 μL |
Carrier: | Hydrogen @ 1.0 mL/min (constant flow) |
Oven Program: | 35 °C for 2.5 min to 85 °C @ 10 °C/min and hold until last peak elutes |
Sample:
- Methyl Formate
- Acetone
- Ethyl Acetate
- Methyl Ethyl Ketone
- Methanol
- 2-Methyl-2-propanol
- Methylene Chloride
- Benzene
- Ethanol
- 2-Butanol
- Toluene
- n-Propanol
- Ethyl Benzene
- p-Xylene
- m-Xylene
- 1-Butanol
- o-Xylene
Faster analysis
ZB-WAXPLUS™ columns provide high efficiency with stable selectivity in a range of dimensions, so you can easily optimize your column dimensions and run up to 65 % faster.
Conditions same for both columns:
Column: | Zebron ZB-WAXPLUS |
Dimension: | As listed |
Detection: | MSD; 45-450 amu |
Part No.: | A) 7KG-G013-11B) 7CB-G013-02 |
Injection: | A) Split 40:1 @ 220 °C, 0.1 μLB) Split 20:1 @ 220 °C, 0.2 μL |
Carrier: | A) Helium @ 1.2 mL/min (constant flow)B) Helium @ 0.3 mL/min (constant flow) |
Oven Program: | A) 40 °C for 0.2 min to 210 °C @ 10 °C/min for 10 min B) 35 °C for 1 min to 250 °C @ 30 °C/min for 5 min |
Sample
1. α-Pinene
2. β-Pinene
3. Sabinene
4. 3-Carene
5. β-Myrcene
6. Limonene
7. β-Phellandrene
8. Octanal
9. Nonanal
10. Limonene Oxides
11. Citronellal
12. Decanal
13. α-Cubebene
14. Linalool
15. β-Cubebene
16. Octanol
17. Germacrene
18. Caryophyllene
19. trans-p-Mentha-2,8-dienol
20. cis-p-Mentha-2,8-dienol
21. Gerenial
22. α-Terpineol
23. Dodecanal
24. Valencene
25. Citral
26. Carvone
27. Cadinene
28. Perillaldehyde
29. trans-Carveol
30. cis-Carveol
31. Perillol
32. Octanoic acid
Documents
- Brochures: Zebron PLUS GC Column Product Brochure | Phenomenex
- Guide: GC Troubleshooting Quick Reference Guide
- Guide: Zebron GC Column Selection Chart
- Brochures: Zebron Gas Management Brochure
- Supporting Docs: Zebron Gas Filter User Guide
- Supporting Docs: Zebron Gas Filter Selection Tool
- Guide: Analyzing Fats and Oils by GC
- Brochures: Application Handbook, Chromatography Solutions for API Manufacturers
- Brochures: Chemical Industry LC & GC Separation Solutions Guide
- Technical Notes: Robust Analysis of Nitrosamines in a Losartan Drug Substance using Strata Activated Carbon Extraction
FAQs
Can I pack or re-pack my GC liner with wool?
Though the upfront cost of self-packing your liner may seem attractive, the time and headaches caused by resulting tailing or irreproducible peaks can be sizeable! Self-packed wool fibers commonly break during installation and any existing deactivation on the liner can also be scratched or damaged. Scratches in the deactivation layer are likely to occur when removing wool from the liner or re-packing it with new wool. Also, when wool is forcefully inserted or removed, these scratches could expose active sites, resulting in peak tailing and poor reproducibility. Pre-packed Zebron PLUS liners undergo the deactivation process with the quartz wool already in place, which ensures that any active sites that form during packing are not exposed. Its novel touchless packaging and preinstalled O-ring prevent contamination during installation.
What are the differences between the ZB-Wax and ZB-WaxPlus?
The ZB-WaxPlus phase is made of a lower molecular weight polymer that has liquid character at lower temperature than the original ZB-Wax. The unique bonding of the lower molecular weight PEG polymer in ZB-WaxPlus allows it to be used with 100% aqueous samples, without inconsistencies from injection to injection, and gives better selectivity for low boiling point compounds. The ZB-WaxPlus tends to give better peak shape for acids while the original ZB-Wax gives better peak shape for basic compounds.
Does the Zebron PLUS liner with wool improve my GC column lifetime?
Yes. Zebron PLUS liners are packed with quartz wool, which serves two purposes: a) Quartz wool promotes homogenous vaporization of analytes and solvent in the inlet to effectively transfer them to the head of the GC column. b) Quartz wool acts as a filter, trapping non-volatile impurities and preventing them from proceeding further into the GC column.
What effects do water and oxygen have on GC column lifetime?
In general, water does not have an impact on capillary columns containing chemically bonded polysiloxane stationary phase. However, polyethylene glycol (PEG) based phases may be affected by repeated injections of water that can result in a change in column polarity. For injections of water samples, a Zebron ZB-WAXplus GC column is recommended. This column will not change polarity or peak shape even if solvent rinsed with water. All columns run at low isothermal temperatures may retain water, affecting peak shape. Routinely elevating the column temperature will remove the water and restore column performance. Exposure to oxygen, particularly at elevated temperatures, will result in irreversible column damage. This is usually seen as excessive bleed levels but may also present as differences in selectivity and peak shape. To avoid possible damage, combination oxygen/moisture traps (AG0-4778 or AG0-4779), are typically used to reduce oxygen and water levels to < 15 ppb.
I work with dirty samples which produce a residue on my GC liner after approximately 100 injections. Can I scrub/sonicate my liner and reuse it?
It is not recommended to scrub/sonicate your liners. Residue formation indicates contamination. More specifically, this shows that the sample contains non-volatile impurities, in which case it is recommended to replace your liner rather than clean it. Sonication can alter the liner’s deactivation, and scrubbing can create scratches and active sites. As a result, you can have several chromatography problems such as poor quantitation, analyte breakdown, poor reproducibility, peak asymmetry, etc.
Is there a way I can get immediate help with my current and upcoming method?
Absolutely, we're always here to help! You can easily get in contact with our team members by clicking contact us on the menu or better yet, just head to www.phenomenex.com/chatnow and we'll help you out with method recommendations and tips.
I am using an inert GC column for my analysis, and I still see peak tailing. What could be the reason for this?
For analysis of active compounds, it is crucial to use both a highly inert column and a highly inert liner. The liner is the first place of potential analyte interaction during GC analysis; it is important that your analytes are not adsorbed. Zebron PLUS liners undergo a rigorous deactivation process and are tested for inertness to ensure reliable results when working with highly active compounds such as underivatized acids and active bases.
When facing peak and resolution issues in sample analysis by GC, where is the best place to start?
The inlet is the most common place where problems happen in gas chromatography. This is because: • The inlet is subjected to very high temperatures. • There are multiple consumables involved in the inlet that need routine maintenance. • Some inlet modes, such as splitless injection, are a slow process, meaning that target analytes and the inlet can interact with each other before the sample enters the column.
Can you recommend a GC liner that work best when analyzing dirty samples, samples with wide boiling points, or samples with wide molecular weight?
Analysis of dirty samples, samples with wide boiling points, or samples with wide molecular weights can be enhanced by choosing a Zebron™ PLUS liner with quartz wool. Liners packed with wool can prevent non-volatile compounds from entering the column and improve sample vaporization for a more robust, reproducible analysis.
Manufacturer information: Zebron ZB-WAXplus GC Columns – Phenomenex (USA). MAS Brand is the official representative of Phenomenex in Egypt.
Specifications for Zebron ZB-WAXplus GC Columns
| GC Length (m) | 10 or 15 or 20 or 25 or 30 or 50 or 60 or N/A |
| GC Internal Diameter (mm) | 0.1 or 0.18 or 0.25 or 0.32 or 0.53 or N/A |
| GC Film Thickness (µm) | 0.1 or 0.15 or 0.18 or 0.19 or 0.25 or 0.5 or 1 or 2 or 3 or N/A |
| GC Format | Capillary Column or 5-inch Cage |
| GC Option | Standard or Custom or w/ GUARDIAN 5 m, Custom |
| Manufacturer | Phenomenex |
| GC Phase | ZB-WAXplus |
