Zebron ZB-Bioethanol GC Columns
Fast, Accurate Denatured Fuel Ethanol Analysis
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Zebron ZB-Bioethanol GC Columns
Fast Quality Checks for Bioethanol Testing
- Specially designed for fast and accurate bioethanol analysis
- Meet ASTM D5501 requirements - resolve methanol and ethanol from all other denaturant peaks
- Great resolution of fusel alcohols
- Allows for quick bake out between runs to eliminate contaminants
Doing biodiesel testing? See ZB-1HT or ZB-5HT.
Recommended Use
Alcohol, Bioethanol, Fusel Alcohols
Overview
Upgrade to Zebron from traditional phases used for bioethanol:
Agilent® | Restek® | SGE® | Supelco® |
|---|---|---|---|
DB®-1 | Rtx®-1 | BP1 | SPB®-1 |
| HP-1 | Rxi®-1ms | SE-30 | |
| CP-Sil 5 CB |
Zebron ZB-Bioethanol
- Resolve methanol and ethanol from all other denaturant peaks
- With analysis time down to 5 minutes, complete certificate of analysis (COA) before fuel truck leaves
- Major cost savings over the standard 100 meter GC columns
Zebron ZB-Bioethanol columns provide bioethanol analysis in less than half the time of standard GC columns. Using this new, specially designed phase, resolution of methanol and ethanol from a sample denatured with gasoline can be achieved in less than 8 minutes. In fact, the column provides such great resolution that the analysis can be reduced to less than 5 minutes by using a 15 meter column. This dramatic time savings gives you the ability to test the fuel trucks before they leave the facility reducing the chance for potential problems.
ASTM Method D 5501: Determination of Denatured Bioethanol
Column: | Zebron ZB-1 |
Dimension: | A) 100 meter x 0.25 mm x 0.50 µm |
Part No.: | |
Injection: | Split 50:1 @ 300 °C, 1 µL |
Carrier Gas: | Helium @ 35 cm/sec (Constant Flow) |
Oven Program: | 45 °C for 7 min to 255 °C @ 30 °C/min(Hold 6 min) |
Detector: | FID @ 300 °C |
Instrument: | Shimadzu® GC-2010 with Flame Ionization, Detection and AOC-20i Automatic Liquid |
Sample:
1. Methanol
2. Ethanol
3. Denaturant
ASTM Method D 5501: Determination of Denatured Bioethanol
Column: | Zebron ZB-Bioethanol |
Dimension: | B) 30 meter x 0.25 mm x 1.00 µm C) 15 meter x 0.25 mm x 1.00 µm |
Part No.: | B) 7HG-G020-22 |
Injection: | Split 50:1 @ 300 °C, 1 µL |
Carrier Gas: | B) Hydrogen @ 35 cm/sec (Constant Flow)C) Hydrogen @ 25 cm/sec (Constant Flow) |
Oven Program: | B) 45 °C for 2.5 min to 255 °C @ 30 °C/min (Hold 4.5 min)C) 55 °C for 1.7 min to 260 °C @ 40 °C/min (Hold 2.67 min) |
Detector: | FID @ 300 °C |
Instrument: | Shimadzu® GC-2010 with Flame Ionization Detection and AOC-20i Automatic Liquid |
Sample:
1. Methanol
2. Ethanol
3. Denaturant
Great Resolution of Fuel Alcohols Products
Since gasoline is a very complex mixture and contains many low boiling components that could potentially co-elute with methanol and ethanol, the resolution provided by the Zebron™ ZB-Bioethanol column was confirmed using GC-MS. As you can see, the methanol and ethanol peaks are well resolved from all potential interferences.
Column: | Zebron ZB-Bioethanol |
Dimension: | 30 meter x 0.25 mm x 1.00 μm |
Part No.: | |
Injection: | Split 100:1 @ 240 °C, 0.1 μL |
Carrier Gas: | Helium @ 1.2 mL/min (Constant Flow) |
Oven Program: | 40 °C for 5 min to 300 °C @ 25 °C/min |
Detector: | MSD @ 230 °C; 30 - 450 amu |
Sample:
1. Methanol
2. Ethanol
3. Acrolein
4. Acetone
5. 2-Methylbutane
6. Isopropyl alcohol
7. Pentane
8. t-Butanol
9. Allyl alcohol
10. n-Propanol
11. 2,3-Dimethylbutane
12. 2-Methylpentane
13. 3-Methylpentane
14. 2-Butanol
15. Ethyl acetate
16. Hexane
17. Methylcyclopentane
18. 2,4-Dimethylpentane
19. Benzene
20. Cyclohexane
21. 2-Methylhexane
22. 2,3-Dimethylpentane
23. 3-Methylhexane
24. 2,2,4-Trimethylpentane
25. Heptane
26. Acetal
27. Toluene
28. Xylene
29. Trimethylbenzene
Compatible with Various Carrier Gases
As competition in the biofuels market increases, the need to reduce costs while maintaining quality is of major concern. The shortages of helium and its increasing costs have forced many labs to consider using alternative gases such as hydrogen.
The Zebron™ ZB-Bioethanol column is compatible with all carrier gases and will provide great separation no matter which gas you choose. To verify this, we ran several denatured fuel ethanol samples using various carrier gases. The results were virtually the same regardless of the carrier gas, proving the selectivity provided by the Zebron™ ZB-Bioethanol was not affected.
No resolution changes using different carrier gases
Column: | Zebron ZB-Bioethanol |
Dimension: | 15 meter x 0.25 mm x 1.00 μm |
Part No.: | |
Injection: | Split 50:1 @ 300 °C, 1 μL |
Carrier Gas: | A) Hydrogen @ 25 cm/sec (Constant Flow)B) Helium @ 25 cm/sec (Constant Flow) |
Oven Program: | 55 °C for 1.7 min to 260 °C @ 40 °C/min (Hold 2.67 min) |
Detector: | FID @ 300 °C |
Instrument: | Shimadzu® GC-2010 with a AOC-20i |
Sample:
1. Methanol
2. Ethanol
3. Denaturant
Documents
- Guide: GC Troubleshooting Quick Reference Guide
- Brochures: Zebron Gas Management Brochure
- Supporting Docs: Zebron Gas Filter Selection Tool
- Supporting Docs: Zebron Gas Filter User Guide
- Guide: Zebron GC Column Selection Chart
FAQs
Can I pack or re-pack my GC liner with wool?
Though the upfront cost of self-packing your liner may seem attractive, the time and headaches caused by resulting tailing or irreproducible peaks can be sizeable! Self-packed wool fibers commonly break during installation and any existing deactivation on the liner can also be scratched or damaged. Scratches in the deactivation layer are likely to occur when removing wool from the liner or re-packing it with new wool. Also, when wool is forcefully inserted or removed, these scratches could expose active sites, resulting in peak tailing and poor reproducibility. Pre-packed Zebron PLUS liners undergo the deactivation process with the quartz wool already in place, which ensures that any active sites that form during packing are not exposed. Its novel touchless packaging and preinstalled O-ring prevent contamination during installation.
Does the Zebron PLUS liner with wool improve my GC column lifetime?
Yes. Zebron PLUS liners are packed with quartz wool, which serves two purposes: a) Quartz wool promotes homogenous vaporization of analytes and solvent in the inlet to effectively transfer them to the head of the GC column. b) Quartz wool acts as a filter, trapping non-volatile impurities and preventing them from proceeding further into the GC column.
I work with dirty samples which produce a residue on my GC liner after approximately 100 injections. Can I scrub/sonicate my liner and reuse it?
It is not recommended to scrub/sonicate your liners. Residue formation indicates contamination. More specifically, this shows that the sample contains non-volatile impurities, in which case it is recommended to replace your liner rather than clean it. Sonication can alter the liner’s deactivation, and scrubbing can create scratches and active sites. As a result, you can have several chromatography problems such as poor quantitation, analyte breakdown, poor reproducibility, peak asymmetry, etc.
Is there a way I can get immediate help with my current and upcoming method?
Absolutely, we're always here to help! You can easily get in contact with our team members by clicking contact us on the menu or better yet, just head to www.phenomenex.com/chatnow and we'll help you out with method recommendations and tips.
I am using an inert GC column for my analysis, and I still see peak tailing. What could be the reason for this?
For analysis of active compounds, it is crucial to use both a highly inert column and a highly inert liner. The liner is the first place of potential analyte interaction during GC analysis; it is important that your analytes are not adsorbed. Zebron PLUS liners undergo a rigorous deactivation process and are tested for inertness to ensure reliable results when working with highly active compounds such as underivatized acids and active bases.
When facing peak and resolution issues in sample analysis by GC, where is the best place to start?
The inlet is the most common place where problems happen in gas chromatography. This is because: • The inlet is subjected to very high temperatures. • There are multiple consumables involved in the inlet that need routine maintenance. • Some inlet modes, such as splitless injection, are a slow process, meaning that target analytes and the inlet can interact with each other before the sample enters the column.
Can you recommend a GC liner that work best when analyzing dirty samples, samples with wide boiling points, or samples with wide molecular weight?
Analysis of dirty samples, samples with wide boiling points, or samples with wide molecular weights can be enhanced by choosing a Zebron™ PLUS liner with quartz wool. Liners packed with wool can prevent non-volatile compounds from entering the column and improve sample vaporization for a more robust, reproducible analysis.
Manufacturer information: Zebron ZB-Bioethanol GC Columns – Phenomenex (USA). MAS Brand is the official representative of Phenomenex in Egypt.
Specifications for Zebron ZB-Bioethanol GC Columns
| GC Length (m) | 15 or 30 |
| Manufacturer | Phenomenex |
| GC Phase | ZB-Bioethanol |
| GC Internal Diameter (mm) | 0.25 |
| GC Film Thickness (µm) | 1 |
| GC Format | Capillary Column |
| GC Option | Standard |
