Zebron ZB-BAC-2 GC Columns
Fast, Accurate Blood Alcohol Analysis
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Zebron ZB-BAC-2 GC Columns
Fast, Accurate Blood Alcohol Analysis
- Proprietary column set specially designed for accurate blood alcohol analysis — enhance resolution of ethanol and acetone peaks
- Resolves t-butanol and n-propanol for greater selection of internal standards
- Fast run time with baseline resolution of key components in just 2 minutes
- Achieve dual column confirmation - get two elution order changes with ZB-BAC-1
Need an alternate selectivity for blood alcohol confirmation? See ZB-BAC-1.
Testing drugs of abuse? See ZB-Drug-1.
Recommended Use
Abused Inhalant Anesthetics, Blood Alcohol Analysis
Overview
Upgrade to Zebron from these similar* phases:
Agilent® | Restek® |
|---|---|
DB®-ALC1 | Rtx®-BAC1 |
| DB-ALC2 | Rtx-BAC2 |
| Rtx-VMS |
Faster, More Sensitive Blood Alcohol Analysis
Column: | As listed |
Dimension: | As listed |
Part No.: | |
Injection: | Split 0.8:1 @ 150 °C, 1 mL |
Carrier Gas: | Helium @ 80 cm/sec (constant flow) |
Oven Program: | 40 °C (Isothermal) |
Detector: | FID @ 250 °C |
Sample:
Analytes 0.025 % and internal standards
0.100 % in water
1. Methanol
2. Acetaldehyde
3. Ethanol
4. Isopropanol
5. Acetone
6. t-Butanol (IS)
7. n-Propanol (IS)
8. 2-Butanol (IS)
Column: | As listed |
Dimension: | As listed |
Injection: | Split 5:1 @ 150 °C, 1 mL |
Carrier Gas: | Helium @ 80 cm/sec (constant flow) |
Oven Program: | 40 °C (Isothermal) |
Detector: | FID @ 220 °C |
Sample:
Analytes and internal standards
0.100 % in water
1. Methanol
2. Acetaldehyde
3. Ethanol
4. Isopropanol
5. Acetone
6. t-Butanol (IS)
7. n-Propanol (IS)
8. 2-Butanol (IS)
In contrast, the Phenomenex Zebron ZB-BAC-1 & and ZB-BAC-2 columns were able to fully resolve both t-Butanol and 2-Butanol from other target analytes, while maintaining rapid analysis times. Quantitative analysis on the Zebron BAC pair showed equivalent performance between all three internal standards (n-Propanol, t-Butanol, and 2-Butanol). The LOD & and LOQ for this column set were far below the control limits required by current testing regulations.
Estimated Limits of Detection (LOD) and Quantitation (LOQ) on Zebron ZB-BAC1 and ZB-BAC2 phases. The LODs and LOQs are also displayed in units of ppm in addition to % blood alcohol content.
Compound | Zebron ZB-BAC1 | Zebron ZB-BAC2 | ||
|---|---|---|---|---|
| LOD | LOQ | LOD | LOQ | |
Methanol | 0.0003 %(2.9 ppm) | 0.001 %(9.7 ppm) | 0.0003 %(3.0 ppm) | 0.001 %(10.1 ppm) |
Acetaldehyde | 0.00002 %(0.2 ppm) | 0.00008 %(0.8 ppm) | 0.00002 %(0.2 ppm) | 0.00006 %(0.6 ppm) |
Ethanol | 0.0003 %(2.6 ppm) | 0.0009 %(8.8 ppm) | 0.0002 %(2.2 ppm) | 0.0007 %(7.4 ppm) |
Isopropanol | 0.0002 %(1.8 ppm) | 0.0006 %(5.8 ppm) | 0.0001 %(1.4 ppm) | 0.0005 %(4.6 ppm) |
Acetone | 0.00008 %(0.8 ppm) | 0.0003 %(2.6 ppm) | 0.00005 %(0.5 ppm) | 0.0002 %(1.7 ppm) |
High reproducibility of Zebron ZB-BAC1 and ZB-BAC2 for blood alcohols. Relative RSD values for replicate injections (n=5) were taken at the lowest concentration in the calibration curve of 0.025 %.
Compound | Peak Area % RSD (Absolute) | Peak Area % RSD (Relative to t-Butanol) | Peak Area % RSD (Relative to n-Propanol) | Peak Area % RSD (Relative to 2-Butanol) |
|---|---|---|---|---|
| Methanol | 3.1 | 2.8 | 2.7 | 2.6 |
| Acetaldehyde | 2.1 | 1.6 | 1.5 | 1.4 |
| Ethanol | 3.4 | 2.8 | 2.7 | 2.6 |
| Isopropanol | 3.2 | 2.6 | 2.5 | 2.4 |
| Acetone | 2.2 | 1.6 | 1.5 | 1.4 |
Compound | Peak Area % RSD (Absolute) | Peak Area % RSD (Relative to t-Butanol) | Peak Area % RSD (Relative to n-Propanol) | Peak Area % RSD (Relative to 2-Butanol) |
|---|---|---|---|---|
| Methanol | 2.1 | 1.6 | 1.4 | 1.3 |
| Acetaldehyde | 3.1 | 2.7 | 2.5 | 2.4 |
| Ethanol | 3.2 | 2.7 | 2.5 | 2.4 |
| Isopropanol | 2.2 | 1.6 | 1.4 | 1.3 |
| Acetone | 3.2 | 2.7 | 2.5 | 2.4 |
Documents
- Guide: GC Troubleshooting Quick Reference Guide
- Guide: Zebron GC Column Selection Chart
- Brochures: Zebron Gas Management Brochure
- Supporting Docs: Zebron Gas Filter Selection Tool
- Supporting Docs: Zebron Gas Filter User Guide
FAQs
Can I pack or re-pack my GC liner with wool?
Though the upfront cost of self-packing your liner may seem attractive, the time and headaches caused by resulting tailing or irreproducible peaks can be sizeable! Self-packed wool fibers commonly break during installation and any existing deactivation on the liner can also be scratched or damaged. Scratches in the deactivation layer are likely to occur when removing wool from the liner or re-packing it with new wool. Also, when wool is forcefully inserted or removed, these scratches could expose active sites, resulting in peak tailing and poor reproducibility. Pre-packed Zebron PLUS liners undergo the deactivation process with the quartz wool already in place, which ensures that any active sites that form during packing are not exposed. Its novel touchless packaging and preinstalled O-ring prevent contamination during installation.
Does the Zebron PLUS liner with wool improve my GC column lifetime?
Yes. Zebron PLUS liners are packed with quartz wool, which serves two purposes: a) Quartz wool promotes homogenous vaporization of analytes and solvent in the inlet to effectively transfer them to the head of the GC column. b) Quartz wool acts as a filter, trapping non-volatile impurities and preventing them from proceeding further into the GC column.
I work with dirty samples which produce a residue on my GC liner after approximately 100 injections. Can I scrub/sonicate my liner and reuse it?
It is not recommended to scrub/sonicate your liners. Residue formation indicates contamination. More specifically, this shows that the sample contains non-volatile impurities, in which case it is recommended to replace your liner rather than clean it. Sonication can alter the liner’s deactivation, and scrubbing can create scratches and active sites. As a result, you can have several chromatography problems such as poor quantitation, analyte breakdown, poor reproducibility, peak asymmetry, etc.
Is there a way I can get immediate help with my current and upcoming method?
Absolutely, we're always here to help! You can easily get in contact with our team members by clicking contact us on the menu or better yet, just head to www.phenomenex.com/chatnow and we'll help you out with method recommendations and tips.
I am using an inert GC column for my analysis, and I still see peak tailing. What could be the reason for this?
For analysis of active compounds, it is crucial to use both a highly inert column and a highly inert liner. The liner is the first place of potential analyte interaction during GC analysis; it is important that your analytes are not adsorbed. Zebron PLUS liners undergo a rigorous deactivation process and are tested for inertness to ensure reliable results when working with highly active compounds such as underivatized acids and active bases.
When facing peak and resolution issues in sample analysis by GC, where is the best place to start?
The inlet is the most common place where problems happen in gas chromatography. This is because: • The inlet is subjected to very high temperatures. • There are multiple consumables involved in the inlet that need routine maintenance. • Some inlet modes, such as splitless injection, are a slow process, meaning that target analytes and the inlet can interact with each other before the sample enters the column.
Can you recommend a GC liner that work best when analyzing dirty samples, samples with wide boiling points, or samples with wide molecular weight?
Analysis of dirty samples, samples with wide boiling points, or samples with wide molecular weights can be enhanced by choosing a Zebron™ PLUS liner with quartz wool. Liners packed with wool can prevent non-volatile compounds from entering the column and improve sample vaporization for a more robust, reproducible analysis.
Manufacturer information: Zebron ZB-BAC-2 GC Columns – Phenomenex (USA). MAS Brand is the official representative of Phenomenex in Egypt.
Specifications for Zebron ZB-BAC-2 GC Columns
| GC Internal Diameter (mm) | 0.32 or 0.53 |
| GC Film Thickness (µm) | 1.2 or 2 |
| GC Option | Standard or Custom |
| Manufacturer | Phenomenex |
| GC Phase | ZB-BAC-2 |
| GC Length (m) | 30 |
| GC Format | Capillary Column |
