Zebron ZB-5plus GC Columns
Inert, Low Bleed 5% Phenyl Selectivity
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Zebron ZB-5PLUS GC Columns
Inert, Low Bleed 5% Phenyl Selectivity
- Highly inert for improved peak shape of acidic/basic compounds, drugs of abuse, and pesticides
- MS certified low bleed levels provide maximum sensitivity
- Industry leading QC specifications ensure column-to-column performance
- Traditional bonding chemistry provides the same selectivity as other 5% phenyl columns
Running semivolatiles, PAHs, or PBDEs? See ZB-SemiVolatiles.
Testing for drugs of abuse? See ZB-Drug-1.
Recommended Use
Drugs, EPA Methods, FAMEs, Nitrosamines, Pesticides, Phenols
Overview
Zebron PLUS GC Columns
Improved Inertness, Better Peak Shape, Low Bleed, and Aqueous Stability
Active sites on a GC column’s surface can result in analyte adsorption and degradation, which negatively affect peak shape and response. To reduce potential surface activity, Zebron PLUS phases (ZB-1PLUS, ZB-5PLUS, ZB-5MSPLUS, and ZB-624PLUS) are designed with a rigorous fused silica deactivation process that improves inertness and peak shapes for troublesome compounds. Zebron ZB-WAXPLUS is specially designed for 100% aqueous stability, enhanced resolution and better reproducibility.
A. Low bleed phase chemistry
B. Clean, highly inert fused silica surface due to very few active sites
C. Temperature resistant and flexible coating
Equivalent or Better Results
Agilent® | Restek® | SGE® | Supelco® | OV® |
|---|---|---|---|---|
DB®-5 | Rtx®-5 | BP5 | MDN-5 | OV-5 |
| HP-5 | Rtx-5MS | BPX5 | SPB®-5 | |
| HP-5ms | Rtx-5Amine | Equity®-5 | ||
| HP-5msi | Rxi®-5ms |
Phenols
Column: | Zebron ZB-5PLUS |
Dimension: | 30 meter x 0.25 mm x 0.25 µm |
Detector: | MSD @ 230 °C, 45-450 amu |
Part No.: | 7HG-G032-11 |
Injection: | Split 5:1 @ 240 °C, 1 µL |
Carrier Gas: | Helium @ 1.2 mL/ min (constant flow) |
Oven Program: | 60 °C to 140 °C @ 5 °C/min to 280 °C @ 10 °C/min |
Sample:1. Phenol
2. 2-Chlorophenol
3. 2-Methylphenol
4. 4-Methylphenol
5. 3-Methylphenol
6. 2,6-Dimethylphenol
7. 2-Nitrophenol
8. 2-Ethylphenol
9. 2,4-Dimethylphenol
10. 3,5-Dimethylphenol
11. 2,5-Dimethylphenol
12. 4-Ethylphenol
13. 3-Ethylphenol
14. 2,4-Dichlorophenol
Sample:
15. Benzoic Acid
16. 2,3-Dimethylphenol
17. 3,4-Dimethylphenol
18. 2,6-Dichlorophenol
19. 4-Chloro-3-methylphenol
20. 2,4,6-Trichlorophenol
21. 2,4,5-Trichlorophenol
22. 2,4-Dinitrophenol
23. 4-Nitrophenol
24. 2,3,4,6-Tetrachlorophenol
25. 4,6-Dinitro-2-methylphenol
26. Pentachlorophenol
27. Dinosebl
Good Results for Drugs
Drug Screening by GC-MS
Column: | Zebron ZB-5PLUS |
Dimension: | 30 meter x 0.25 mm x 0.25 µm |
Detector: | MSD @ 230 °C, 45-450 amu |
Part No.: | 7HG-G032-11 |
Injection: | Split 15:1 @ 240 °C, 1 µL |
Carrier Gas: | Helium @ 1.1 mL/ min (constant flow) |
Oven Program: | 140 °C to 240 °C @ 10 °C/min for 5 minto 320 °C @ 25 °C/min for 2.25 min |
Sample:
1. Nicotine
2. Methylecgonine
3. Ibuprofen
4. Allobarbital
5. Aprobarbital
6. Butabarbital
7. Acetaminophenl
8. Phenacetin
9. Amobarbital
10. Pentobarbital
11. Secobarbital
12. Meprobamate
13. Methyl benzilate
14. Caffeine
15. Benzphetamine
16. Hexobarbital
Sample:
17. Dimenhydrinate
18. Doxylamine
19. Phenobarbital
20. 8-Chlorotheophylline
21. Methapyrilene
22. Chlorpheniramine
23. Brompheniramine
24. Cocaine
25. Chlorcyclizine
26. Codeine
27. Morphine
28. Diazepam
29. Hydrocodone
30. 6-Monoacetylmorphine
31. Oxymorphone
32. Diacetylmorphine (Heroin)
Endocrine Disruptors by GC-MS
Column: | Zebron ZB-5PLUS |
Dimension: | 30 meter x 0.25 mm x 0.25 µm |
Detector: | MSD @ 180 °C, 45-450 amu |
Part No.: | 7HG-G032-11 |
Injection: | Split 40:1 @ 250 °C, 1 µL |
Carrier Gas: | Helium @ 1.2 mL/min (constant flow) |
Oven Program: | 100 °C to 180 °C @ 5 °C/min to 320 °C @ 15 °C/min |
Sample:
Analytes are 50 ppm in acetone
1. Dimethyl phthalate
2. Diethyl phthalate
3. Atraton
4. Simazine
5. Prometon
6. Atrazine
7. Propazine
8. Dipropyl phthalate
9. Terbuthylazine
10. Secbumetone
11. Simetryn
Sample:12. Ametryn
13. Prometryn
14. Terbutryn
15. Dibutyl phthalate
16. 4,4’-DDE
17. 4,4’-DDD
18. Di-n-hexyl phthalate
19. 4,4’-DDT
20. Dicyclohexyl phthalate
21. bis(2-Ethylhexyl)phthalate
22. Di-n-octyl phthalate
23. Ethinyl estradiol
Documents
- Guide: GC Troubleshooting Quick Reference Guide
- Guide: Zebron GC Column Selection Chart
- Brochures: Zebron Gas Management Brochure
- Technical Notes: Extraction and Analysis of THC and Metabolites from Whole Blood and Urine by GC-MS (TN-2062)
- Brochures: How To Choose A 5 Phase Column
- Supporting Docs: Zebron Gas Filter User Guide
- Supporting Docs: Zebron Gas Filter Selection Tool
- Technical Notes: Analysis of 33 Primary and Secondary Terpenes Found in Cannabis by GC-FID
- Brochures: Zebron PLUS GC Column Product Brochure | Phenomenex
FAQs
Can I pack or re-pack my GC liner with wool?
Though the upfront cost of self-packing your liner may seem attractive, the time and headaches caused by resulting tailing or irreproducible peaks can be sizeable! Self-packed wool fibers commonly break during installation and any existing deactivation on the liner can also be scratched or damaged. Scratches in the deactivation layer are likely to occur when removing wool from the liner or re-packing it with new wool. Also, when wool is forcefully inserted or removed, these scratches could expose active sites, resulting in peak tailing and poor reproducibility. Pre-packed Zebron PLUS liners undergo the deactivation process with the quartz wool already in place, which ensures that any active sites that form during packing are not exposed. Its novel touchless packaging and preinstalled O-ring prevent contamination during installation.
Does the Zebron PLUS liner with wool improve my GC column lifetime?
Yes. Zebron PLUS liners are packed with quartz wool, which serves two purposes: a) Quartz wool promotes homogenous vaporization of analytes and solvent in the inlet to effectively transfer them to the head of the GC column. b) Quartz wool acts as a filter, trapping non-volatile impurities and preventing them from proceeding further into the GC column.
I work with dirty samples which produce a residue on my GC liner after approximately 100 injections. Can I scrub/sonicate my liner and reuse it?
It is not recommended to scrub/sonicate your liners. Residue formation indicates contamination. More specifically, this shows that the sample contains non-volatile impurities, in which case it is recommended to replace your liner rather than clean it. Sonication can alter the liner’s deactivation, and scrubbing can create scratches and active sites. As a result, you can have several chromatography problems such as poor quantitation, analyte breakdown, poor reproducibility, peak asymmetry, etc.
Is there a way I can get immediate help with my current and upcoming method?
Absolutely, we're always here to help! You can easily get in contact with our team members by clicking contact us on the menu or better yet, just head to www.phenomenex.com/chatnow and we'll help you out with method recommendations and tips.
I am using an inert GC column for my analysis, and I still see peak tailing. What could be the reason for this?
For analysis of active compounds, it is crucial to use both a highly inert column and a highly inert liner. The liner is the first place of potential analyte interaction during GC analysis; it is important that your analytes are not adsorbed. Zebron PLUS liners undergo a rigorous deactivation process and are tested for inertness to ensure reliable results when working with highly active compounds such as underivatized acids and active bases.
When facing peak and resolution issues in sample analysis by GC, where is the best place to start?
The inlet is the most common place where problems happen in gas chromatography. This is because: • The inlet is subjected to very high temperatures. • There are multiple consumables involved in the inlet that need routine maintenance. • Some inlet modes, such as splitless injection, are a slow process, meaning that target analytes and the inlet can interact with each other before the sample enters the column.
Can you recommend a GC liner that work best when analyzing dirty samples, samples with wide boiling points, or samples with wide molecular weight?
Analysis of dirty samples, samples with wide boiling points, or samples with wide molecular weights can be enhanced by choosing a Zebron™ PLUS liner with quartz wool. Liners packed with wool can prevent non-volatile compounds from entering the column and improve sample vaporization for a more robust, reproducible analysis.
Manufacturer information: Zebron ZB-5PLUS GC Columns – Phenomenex (USA). MAS Brand is the official representative of Phenomenex in Egypt.
Specifications for Zebron ZB-5plus GC Columns
| GC Length (m) | 10 or 15 or 20 or 30 or 60 |
| GC Internal Diameter (mm) | 0.18 or 0.25 or 0.32 |
| GC Film Thickness (µm) | 0.1 or 0.18 or 0.25 or 0.5 or 1 |
| GC Format | Capillary Column or 5-inch Cage |
| GC Option | Standard or w/ GUARDIAN 5 m |
| Manufacturer | Phenomenex |
| GC Phase | ZB-5plus |
